Assistant/Associate Project Scientist - Cellular Biology - Molecular & Cell Biology

The Molecular & Cell Biology Department at the University of California, Berkeley seeks applications for a Assistant or Associate Project Scientist in the Harland Lab in the area of developmental biology.

The theme of the current project is to understand the specific functions of the Plekhg family of Rho GTPases in gastrulation of the frog Xenopus. The lab has several projects ongoing, including a late-stage project focused on Plekhg3, where we have found it has an important role regulating cell stiffness during gastrulation. We also found tantalizing evidence for Plekhg7 being important in brain and eye development. In both cases we find that context, the location and timing of expression, is key to Rho GTPase regulation.

Responsibilities:

The candidate will carry out high quality live imaging of embryos during gastrulation or neurulation, fluorescent imaging of cell movements, and quantification of signal through time. The candidate will use laser ablation to quantify cellular properties. Importantly, preparing mRNA, CRISPR, or plasmid reagents, and their injection to influence or track development, is essential to almost all project questions. The mentorship of undergraduate students is encouraged, so leading a team to achieve project goals is expected. This position will contribute to manuscript and grant preparation.

Applicants must be authorized to work in the United States at the time of hire. Visa sponsorship is not available for this position.

PhD or equivalent international degree

At least two years post-PhD research experience.

• PhD in biology or subfield, with preference given to those with demonstrated expertise in developmental biology.
• Experience studying Xenopus morphogenesis, molecular biology, gene expression, or small GTPase regulators is strongly encouraged.
• Expertise with methods such as live confocal imaging, mRNA-seq, in situ hybridization, CRISPR screens, protein biochemistry, and RNA biology, and laser ablation is desirable.
• Excellent writing and communication skills.
• Strong publication record in peer reviewed journals.
• Experience working with and mentoring undergraduate students.

• 3 required (contact information only)

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